| 质粒名称: | pLKO.1 - TRC cloning vector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 10878 |
| 存储实验室: | David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 7032 bp |
| 完整质粒大小: | 8932 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral, RNAi |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | stuffer |
| 插入大小: | 1900 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | LKO.1 5' |
|
文献引用方法 |
|
| 材料和方法部分: | pLKO.1 - TRC cloning vector was a gift from David Root (爱迪基因 plasmid # 10878) |
| 参考文献部分: | A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE. Cell. 2006 Mar 24. 124(6):1283-98. 10.1016/j.cell.2006.01.040 PubMed 16564017 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLKO.1 - TRC cloning vector 8901 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION 3rd generation transfer plasmid.
ACCESSION .
VERSION .
KEYWORDS pLKO.1 - TRC cloning vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8901)
AUTHORS Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G,
Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY,
Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini
DM, Root DE
TITLE A lentiviral RNAi library for human and mouse genes applied to an
arrayed viral high-content screen.
JOURNAL Cell. 2006 Mar 24. 124(6):1283-98.
PUBMED 16564017
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pLKO.1 - TRC cloning vector |
FEATURES Location/Qualifiers
source 1..8901
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 393..413
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 624..857
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 929..1050
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(966..985)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1020..1039
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 1090..1225
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1152..1171
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(1245..1264)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1246..1264)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1274..1291)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1274..1290)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1283..1305)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 1432..1887
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1519..1538)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1729..1750
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 1913..2017
/gene="bla"
/label=AmpR promoter
CDS 2018..2878
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2236..2255)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3049..3637
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3538..3557
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3791..3808
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3925..3946
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3961..3991
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3999..4015
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4004..4026
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4023..4039
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4023..4039
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 4058..4078
/label=T3
/note="T3 promoter, forward primer"
promoter 4060..4078
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 4106..4332
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 4333..4513
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4560..4685
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5178..5411
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 5938..6178
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 5938..5958
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 6109..6128
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_feature 8100..8217
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 8266..8776
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 8695..8713
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 8798..496
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8798..8817)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
ORIGIN
1 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
61 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
121 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
181 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
241 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
301 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
361 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
421 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
481 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
541 accccatgca tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
841 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
901 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1021 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
1081 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
1201 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
1261 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
1321 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
1381 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
1441 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
1501 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
1561 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
1621 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
1681 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
1741 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
1801 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
1861 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
1921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
1981 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
2041 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
2101 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
2161 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
2221 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
2281 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
2341 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
2401 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
2461 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
2521 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
2581 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
2641 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
2701 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
2761 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
2821 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
2881 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
2941 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
3001 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
3061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
3121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
3181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
3241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
3301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
3361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
3421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
3481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
3541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
3601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
3661 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
3721 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
3781 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
3841 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
3901 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
3961 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
4021 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
4081 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
4141 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
4201 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
4261 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
4321 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
4381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
4441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
4501 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
4561 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
4621 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
4681 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
4741 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
4801 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
4861 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
4921 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
4981 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
5041 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
5101 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
5161 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
5221 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
5281 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
5341 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
5401 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
5461 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
5521 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
5581 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
5641 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
5701 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
5761 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
5821 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
5881 aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag
5941 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
6001 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
6061 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
6121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
6181 aacaccggtg tgttgtaaat gagcacacaa aatacacatg ctaaaatatt atattctatg
6241 acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat aattagaatt
6301 tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa cgctagtttt
6361 agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt gttcctaagc
6421 ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact ccaccagctg
6481 ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct tcaaatgttg
6541 cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct tcaacagcta
6601 tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata atacatttaa
6661 caacatgtga tccagctgct ttttttacag ctttcatgtc ttctaaaact aattcataat
6721 ttttgtcttt taatgcacca atatttaata ccatatcaat ttctgttgca ccatctttaa
6781 ttgcttcaga aacttcgaat gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta
6841 caacatttgt tattcctaca tttgtgcctt ttaataattc tttacaatag cttgttcaat
6901 atgaattaac acaaactgtt gcaaaatcaa attcaattgc ttcatcacat aattgtttaa
6961 tttcagcttt cgtagcatct tgttttaata atgtgtgatc tatatatttg tttagtttca
7021 ttttttctcc tatatattca tttttaattt taattcttta ataatttcgt ctactttaac
7081 tttagcgttt tgaacagatt caccaacacc tataaaataa atttttagtt taggttcagt
7141 tccacttggg cgaacagcaa atcatgactt atcttctaaa taaaatttta gtaagtcttg
7201 tcctggcata ttatacattc catcgatgta gtcttcaaca ttaacaactt taagtccagc
7261 aatttgagtt aagggtgttg ctctcaatga tttcattaat ggttcaattt ttaatttctt
7321 ttcttctggt ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca tttctttaaa
7381 taaatcttct aaatagtcta ctaatgtttt attttgtttt ttataaaatc aagcagcctc
7441 tgctattaat atagaagctt gtattccatc tttatctcta gctgagtcat caattacata
7501 tccataactt tcttcataag caaaaacaaa atttaatccg ttatcttctt ctttagcaat
7561 ttctctaccc attcatttaa atccagttaa agtttttaca atattaactc catatttttc
7621 atgagcgatt ctatcaccca aatcacttgt tacaaaactt gaatatagag ccggattttt
7681 tggaatgcta tttaagcgtt ttagatttga taattttcaa tcaattaaaa ttggtcctgt
7741 ttgatttcca tctaatctta caaaatgacc atcatgtttt attgccattc caaatctgtc
7801 agcatctggg tcattcataa taataatatc tgcatcatgt ttaataccat attcaagcgg
7861 tatttttcat gcaggatcaa attctggatt tggatttaca acatttttaa atgtttcatc
7921 ttcaaatgca tgctcttcaa cctcaataac gttatatcct gattcacgta atatttttgg
7981 ggtaaattta gttcctgttc cattaactgc gctaaaaata atttttaaat cttttttagc
8041 ttcttgctct tttttgtaga attctcgacc tcgagacaaa tggcagtatt catccacaat
8101 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
8161 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
8221 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
8281 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
8341 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
8401 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
8461 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
8521 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
8581 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
8641 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
8701 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
8761 cgacctctct ccccaggggg atccaccgga gcttaccatg accgagtaca agcccacggt
8821 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
8881 cgactacccc gccacgcgcc a
//
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